美國(guó)BIO-RAD伯樂Trans-Blot?Turbo全能型蛋白轉(zhuǎn)印系統(tǒng)1704150
美國(guó)BIO-RAD伯樂Trans-Blot?Turbo全能型蛋白轉(zhuǎn)印系統(tǒng)1704150 | 時(shí)間: September-1 14:01:57 | 分類:其它儀器
凌儀生物提供高速離心機(jī),美國(guó)Bio-rad伯樂電泳,德國(guó)eppendorf艾本德移液器,美國(guó)ABI基因擴(kuò)增儀PCR,雅培原位雜交儀,Thermo熱電離心機(jī)等實(shí)驗(yàn)室儀器設(shè)備,以及Corning康寧,Axygen愛思進(jìn),Greiner格瑞拉等耗材要省錢,別錯(cuò)過哦。
- 快速轉(zhuǎn)印 - 只需3分鐘即可轉(zhuǎn)印小型凝膠或中型凝膠
- 高通量 - 單次運(yùn)行可轉(zhuǎn)印1-4塊小型膠或1-2塊中型膠
- 更高的轉(zhuǎn)印效率 - 比其他轉(zhuǎn)印方法的轉(zhuǎn)印效率更高
- 靈活設(shè)計(jì) - 允許用戶自定義轉(zhuǎn)移條件并與傳統(tǒng)的半干式消耗品相容
- 環(huán)境友好 - 環(huán)保型耗能節(jié)省廢棄物處理費(fèi)用
與其他印跡技術(shù)相比,使用Trans-Blot®Turbo™系統(tǒng)可以獲得更高的靈敏度和更好的轉(zhuǎn)印效率。這一數(shù)據(jù)集顯示僅使用Trans-Blot Turbo系統(tǒng)成功轉(zhuǎn)印1.25 ng蛋白條帶。在Criterion™TGX™4-20%凝膠上分離轉(zhuǎn)鐵蛋白的系列稀釋并使用四種不同印跡技術(shù)進(jìn)行轉(zhuǎn)移。所有圖像均調(diào)整至相同設(shè)置。
使用Trans-Blot®Turbo™轉(zhuǎn)印包進(jìn)行快速轉(zhuǎn)印
3分鐘方案 - 在短短3分鐘內(nèi)完成Mini-PROTEAN®TGX™凝膠的超快轉(zhuǎn)轉(zhuǎn)(蛋白分子量范圍:5-150 kD)
7分鐘方案 - 在7分鐘內(nèi)高效轉(zhuǎn)印4塊小型或2塊中型凝膠(蛋白質(zhì)分子量范圍:5-150 kD)
10分鐘方案 - 在10分鐘內(nèi)高效轉(zhuǎn)印4塊小型或2塊中型凝膠體(蛋白質(zhì)分子量范圍:25-300 + kD)
大腸桿菌裂解物(6μg )稀釋了兩倍。樣本用Mini-PROTEAN TGX凝膠進(jìn)行分離,用Trans-Blot Turbo系統(tǒng)進(jìn)行 轉(zhuǎn)移,用SYPRO Ruby進(jìn)行染色,然后在VersaDoc™MP 4000系統(tǒng)上進(jìn)行成像。通道1中的標(biāo)準(zhǔn)品是Precision Plus Protein™未染標(biāo)準(zhǔn)品,頂部帶為250 kD MW。
2x蛋白質(zhì)轉(zhuǎn)移對(duì)使用Trans-Blot®Turbo™和iBlot系統(tǒng)轉(zhuǎn)印的等量2-D凝膠進(jìn)行的定量表明使用Trans-Blot Turbo系統(tǒng)轉(zhuǎn)印和檢測(cè)到的蛋白數(shù)增加了一倍。 Rad的ReadyStrip™11厘米pH 5-8的IPG膠條上聚焦大鼠肝提取物,并將其分離到AnykD™Criterion™TGX™凝膠上。使用Trans-Blot Turbo和iBlot系統(tǒng)轉(zhuǎn)移復(fù)制凝膠,這兩種系統(tǒng)都使用制造商推薦的7分鐘實(shí)驗(yàn)方案。隨后用SYPRO Ruby對(duì)硝化纖維素膜進(jìn)行染色,并在VersaDoc™MP 4000系統(tǒng)上進(jìn)行成像。
Blotting instrument, includes base, 2 cassettes to hold 1–2 midi or up to 4 mini blotting sandwiches, blot roller, and starter consumable kit .
Trans-Blot® Turbo™ Transfer System: 1704150
Blotting instrument, includes base, 2 cassettes to hold 1-2 midi or up to 4 mini blotting sandwiches, blot roller
訂貨信息:
Trans-Blot® Turbo™ Transfer Starter System | Trans-Blot® Turbo™ Transfer System | ||
| Trans-Blot® Turbo™ Mini PVDF Transfer Packs |
| Trans-Blot® Turbo™ RTA Mini PVDF Transfer Kit, for 40 blots |
| Trans-Blot® Turbo™ Midi PVDF Transfer Packs |
| Trans-Blot® Turbo™ RTA Midi PVDF Transfer Kit, for 40 blots |
| Trans-Blot® Turbo™ Mini Nitrocellulose Transfer Packs |
| Trans-Blot® Turbo™ RTA Mini LF PVDF Transfer Kit, for 40 blots |
| Trans-Blot® Turbo™ Midi Nitrocellulose Transfer Packs |
| Trans-Blot® Turbo™ RTA Midi LF PVDF Transfer Kit, for 40 blots |
| Trans-Blot® Turbo™ RTA Mini Nitrocellulose Transfer Kit, for 40 blots |
| Trans-Blot® Turbo™ Cassette |
| Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit, for 40 blots |
| Trans-Blot® Turbo™ Base |
美國(guó)BIO-RAD伯樂Trans-Blot®Turbo全能型蛋白轉(zhuǎn)印系統(tǒng)1704150
耗材試劑套裝:
Use the Trans-Blot Turbo RTA transfer kit for fast, efficient protein transfer with the Trans-Blot Turbo rapid western blotting transfer system.
- Economical transfer option for high-throughput labs
- Optimized buffer, membrane, and filter paper combination
- PVDF membrane reduces nonspecific binding
- For 40 mini-gel transfers; requires assembly
Related Products
View our wide selection of transfer membrane sandwiches for the Trans-Blot Turbo system:
- Preassembled transfer packs for 10 transfers
- Ready-to-assemble kits for 40 transfers
- PVDF, low fluorescence (LF) PVDF, or nitrocellulose membranes
Trans-Blot Turbo | Preassembled Transfer Packs | Ready-to-Assemble Transfer Kits | ||
Membrane Type | Mini | | Mini | |
0.2 µm PVDF | 1704156 | 1704157 | 1704272 | 1704273 |
0.45 µm LF | — | — | 1704274 | 1704275 |
0.2 µm Nitrocellulose | 1704158 | 1704159 | 1704270 | 1704271 |
TGX凝膠訂購(gòu)信息:
| Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl |
| 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl |
| 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl |
| 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl |
| 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl |
| 10% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl |
| 7.5% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl |
| 12% Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 30 µl |
| 10% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl |
| 12% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well, 15 µl |